[Scanning electron microscopic studies of the basal surface of the corneal endothelium and the stromal and endothelial surfaces of Descemet's membrane in rats].

PURPOSE The basal surface of the corneal endothelium and the stromal and endothelial surfaces of Descement's membrane in rats were studied by scanning electron microscopy. METHODS We compared the fine structures of the two surfaces of Descemet's membrane both after sputter-coating with platinum and without sputter-coating. RESULTS Fine structures were made clearly visible without metal coating by heating specimens to 300 degrees C during observation. On the basal surface of th endothelium, pinocytotic vesicles and invagination of the basal cell membrane were visible. After sputter-coating, Descemet's membrane appeared to be composed of granular substances. The size of the granules was larger on the stromal side than on the endothelial side. Descemet's membrane observed without sputter-coating was composed of fine fibrous structures, showing a felt-like appearance on both stromal and endothelial sides. The diameter of the fibers on the stromal surface was thicker than on the endothelial side. CONCLUSION These results may indicate a characteristic difference between the collagen types existing on the two sides of Descemet's membrane.